Coding

Part:BBa_J56001:Design

Designed by: Matt Eames   Group: iGEM06_UCSF   (2006-10-13)


CheW with VM108 mutation for orthogonal interaction with Tar EA400


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 482
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 482
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 482
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 482
    Illegal AgeI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CheW mutated (VM108) to orthogonally interact with part BBa_J56002 (Tar receptor). Includes restriction site AvrII at the terminus. Mutation was originally (see reference) revealed orthogonality for Tsr receptor, not Tar.

Source

Synthesized by DNA 2.0

References

Liu et al, Journal of Bacteriology (1991)